Enzymes and Inhibitors
New England Biolabs, Inc. PaqCI - 1000 units
PaqCI is a Type IIS restriction enzyme with a 7 basepair recognition sequence: CACCTGC(4/8). It is sourced from an E. coli strain that carries the modified cloned PaqCI gene from Paucibacter aquatile. PaqCI requires multiple sites for efficient cleavage -- PaqCI Activator is included for optimal cleavage. This enzyme is supplied with NEB's rCutSmart Buffer and is heat inactivated.
Sino Biological SuperNuclease
SuperNuclease is a recombined Serratia marcescens’ extracellular endonuclease. The sequence is same with Benzonase (Benzonase is a trademark of Merck KGaA, Darmstadt, Germany).
Gold Biotechnology Inc Proteinase K Solution
Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins It cleaves at the carboxylic acid side of aliphatic aromatic or hydrophobic amino acids Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids Proteinase K is also useful for helping to characterize enzymes due to its cleavage specificity This enzyme was designated proteinase K because of its ability to hydrolyze keratin Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH The isoelectric point of proteinase K is 8 9
Gold Biotechnology Inc D Luciferin Sodium Salt 5g
Tested and Certified for in vivo imaging (See Luciferin FAQ in additional information)
Medchemexpress LLC Glycyl-L-proline 7-amido-4-methylcoumarin hydrobromide | 115035-46-6 | MFCD00152092 | 98.7% | 410.26 g·mol⁻¹ | C17H20BrN3O4 | 5 MG
Glycyl-L-proline 7-amido-4-methylcoumarin hydrobromide (CAS 115035-46-6) is a fluorogenic dipeptide substrate used to measure dipeptidyl peptidase IV (DPP-IV) activity. Enzymatic cleavage releases the fluorescent 7-amido-4-methylcoumarin (AMC) reporter, enabling sensitive continuous or end-point assays in biochemical and enzymatic studies. Supplied as an off-white to light yellow solid with high reported purity for research use.
- Specific fluorescent substrate for detecting DPP-IV activity.
- Releases 7-amido-4-methylcoumarin for sensitive fluorescence detection.
- Suitable for continuous or end-point enzyme assays.
- Compatible with standard fluorescence readers (abs/em ~353/442 nm).
- Supplied as a solid with high reported purity for biochemical use.
Ubpbio SUC-LLVY-AMC, 25MG
Fluorescent Peptides/Protein Substrates – SUC-LLVY-AMC (UBPBio catalog# G1100, G1101) – Suc-LLVY-AMC is a fluorogenic substrate for the chymotrypsin – like activity of the 20S and 26S proteasomes. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.
Ubpbio BOC-LRR-AMC, 5MG
Fluorescent Peptides/Protein Substrates – BOC-LRR-AMC (UBPBio catalog# G3100, G3101) – Boc-LRR-AMC is a fluorogenic substrate for the trypsin – like activity of the 20S proteasome. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.
Echelon Biosciences Research Labs PPLases Substrate, calorimetric - 25 mg, CAS#: 70967-97-4
PPLases Substrate, calorimetric - 25mg , Succinyl-Ala-Leu-Pro-Phe-pNA Category/Application: Enzyme Substrates This item is non returnable
Echelon Biosciences Research Labs Tryptase Substrate - 1 mg,
Tryptase Substrate - 1mg , Ac-Orn-Phe-Arg-AMC Category/Application: Enzyme Substrates This item is non returnable
Bioworld Lysozyme, 10 g
Lysozyme Enzyme used in purification protocols of genomic and plasmid DNA to lyse bacterial cells.Ultra Pure GradeSource: Hen Egg White (also called Hen Egg Lysozyme or HEL.)Activity(25 C): >20,000U/mgAlbumin: None detectedSalmonella: None detected